Synthesis and conformational studies of a stable peptidomimetic -hairpin based on a bifunctional diketopiperazine turn inducer
نویسندگان
چکیده
The design, synthesis and conformational studies of a new b-hairpin mimic, 2, are described in this paper. The design towards hairpin 2 is based on the assembly of the bifunctional DKP-1 as a b-turn inducer, a peptidomimetic strand, namely 5-amino-2-methoxybenzhydrazide, to stabilize the formation of b-sheets, and finally a tetrapeptide sequence, GVVI, containing the hydrophobic residues Val, Ile and Gly. The synthesis of hairpin 2 was performed in solution while the formation of the b-hairpin in protic solvents was proven by NMR investigations (H and C chemical shifts, vicinal coupling constants and ROEs) and corroborated by computational studies (Monte-Carlo conformational search, molecular dynamics and DFT calculations).
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